Nutrient Agar 500 g pH 6.8 (2)

Description

Nutrient Agar is a basic culture medium used for maintenance or to check purity of subcultures prior to biochemical orserological tests from water (1) and Dairy (2). This medium may be used as slants or plates for routine work with non-fastidiousorganisms. Nutrient Agar, pH 6.8 has relatively simple formulation which provides the necessary nutrients for the growth ofmany microorganisms which are not very fastidious. Many bacteria have the optimum pH growth range of 6.6 to 7.0. Wetmore and Gochenour (3) maintained cultures of Malleomyces andPseudomonas on Nutrient Agar to which glycerol was added. Greenberg and Cooper (4) employed this medium in cultivation of Staphylococci for the preparation of vaccines and antigens. Nutrient Agar has relatively simple formulation which provides the necessary nutrients for the growth of many microorganisms which are not very fastidious.HM Peptone B contains vitamins, organic nitrogen compounds, salts and little carbohydates (5). Peptone provide amino acids and long chain peptides for the organisms.